Overview of our system

Vector information

Protocols

Protoplast isolation

Gene transfection

Luciferase assay

Western blotting

Results

Transfect efficiency

Gene expression levels of dual expression vectors

Protein interaction

Acknowledgements

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Aknowledgements

The project was supported by the USDA Cooperative State Research, Education and Extension Service - National Research Initiative - Plant Genome Program, award no. 2006-35604-16627, for N.K. We thank for Dr Frédéric Berger at Université Lyon, France and Dr Xuemei Chen at Rutgers University, NJ for the kind gifts of cDNA clones. N.K. thanks Dr Eric Lam at Rutgers University and JCL for their support during an earlier stage of vector constructions.

 

Last Update 8/1/07