Arabidopsis
Fig. Complemented Renilla luciferase activities in Arabidopsis protoplasts expressing histone H2A and H2B
Assay |
PPI signal |
Split luciferase |
18 |
BRET* |
1.04-1.3 |
FRET** |
1.14-1.27 |
*Subramanian et al. 2006 Nat Biotechnol , 19;769-772
**Cheng et al. 2003 Genes&Development 13;334-344,
Churchman et al., 2006 Plant Cell, 18;3145-3157
Split luciferase can detect protein-protein interaction at 10-fold higher than FRET and BRET assays |
Rice
Fig. Complemented Renilla luciferase activities in Rice protoplasts expressing histone proteins and SYP proteins
Protein-protein interactions detected by this method were published in the follwoing papers.