Transfect efficiencies and gene expression
Format
|
protoplast number |
vector amount |
Efficiency
|
standard deviation
|
microtube |
0.5-1.0x104 |
10µg |
44 % |
7 % |
96-well plate |
0.3-0.6x104 |
10µg |
54 % |
7 % |
* Transfection efficiencies were determined by fluorescence microscope observation.? A percent of cell numbers expressing YFP in an observed cells was calculated.
** A sum of two experiments with the pDuExAn1RG vector, expressing a fusion protein YFP: Renilla-luciferase,? and one experiment with the pDuExAn1GL3 vector, expressing a fusion protein YFP: firefly-luciferase, was averaged.
| Renilla luciferase activities in Arabidopsis protoplasts transfected and mesured in a 96-well plate | |
|
|
Large-format gene transfection homogenously expresses transgenes |